This product is an active protein and may elicit a biological response in vivo, handle with caution.
Crossover junction endodeoxyribonuclease ruvC
Holliday junction nuclease ruvC
Holliday junction resolvase ruvC
In Escherichia coli, the RuvA, RuvB and RuvC proteins are required for the late stages of homologous recombination and DNA repair. They are involved in processing the Holliday junction during homologous recombination. RuvA protein binds to both single-stranded and double stranded DNA and enhances ATPase activity of RuvB. RuvA and RuvB promote branch migration whereas RuvC resolves junctions by endonucleolytic cleavage. Moreover RuvAB stimulate Holliday junction resolution by RuvC. The RuvA-RuvB complex interacts with an irregular conformation in damaged DNA and induces conformational changes in DNA using energy provided by ATP hydrolysis, so that it facilitates DNA repair, recombination and error prone replication. RuvABC proteins are responsible for the occurrence of DSBs at arrested replication forks. In cells proficient for RecBC, RuvAB is uncoupled from RuvC and DSBs may be prevented.
Recombinant E. coli RuvC protein (Active) images
SDS-PAGE - Recombinant E. coli RuvC protein (Active) (ab63828)
SDS-PAGE analysis of Recombinant E. coli RuvC protein (ab63828).
SDS-PAGE - RuvC protein (ab63828)
ab63828 on SDS-PAGE (CBB staining). Molecular Weight: ~19 kDa.
References for Recombinant E. coli RuvC protein (Active) (ab63828)
This product has been referenced in:
García-Luis J & Machín F Mus81-Mms4 and Yen1 resolve a novel anaphase bridge formed by noncanonical Holliday junctions. Nat Commun5:5652 (2014).
Read more (PubMed: 25466415) »