Overview

  • Product nameRecombinant E. coli RuvC protein (Active)
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesEscherichia coli

Associated products

Specifications

Our Abpromise guarantee covers the use of ab63828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

    ELISA

    Functional Studies

  • Purity> 90 % SDS-PAGE.

  • FormLiquid
  • Additional notes


    ab63828 can be used for: 1) Studies on the homologous recombination mechanism. 2) To use as an endonuclease which functions specifically to the Holliday structure.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Stable for 12 months at -20°C.

    Preservative: None
    Constituents: 50% Glycerol, 5mM Beta mercaptoethanol, 2mM EDTA, 100mM Sodium chloride, 10mM Tris HCl, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Crossover junction endodeoxyribonuclease ruvC
    • Holliday junction nuclease ruvC
    • Holliday junction resolvase ruvC
  • RelevanceIn Escherichia coli, the RuvA, RuvB and RuvC proteins are required for the late stages of homologous recombination and DNA repair. They are involved in processing the Holliday junction during homologous recombination. RuvA protein binds to both single-stranded and double stranded DNA and enhances ATPase activity of RuvB. RuvA and RuvB promote branch migration whereas RuvC resolves junctions by endonucleolytic cleavage. Moreover RuvAB stimulate Holliday junction resolution by RuvC. The RuvA-RuvB complex interacts with an irregular conformation in damaged DNA and induces conformational changes in DNA using energy provided by ATP hydrolysis, so that it facilitates DNA repair, recombination and error prone replication. RuvABC proteins are responsible for the occurrence of DSBs at arrested replication forks. In cells proficient for RecBC, RuvAB is uncoupled from RuvC and DSBs may be prevented.

Recombinant E. coli RuvC protein (Active) images

  • ab63828 on SDS-PAGE (CBB staining). Molecular Weight: ~19 kDa.

References for Recombinant E. coli RuvC protein (Active) (ab63828)

This product has been referenced in:
  • García-Luis J & Machín F Mus81-Mms4 and Yen1 resolve a novel anaphase bridge formed by noncanonical Holliday junctions. Nat Commun 5:5652 (2014). Read more (PubMed: 25466415) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"