Recombinant E. coli slyD protein (ab168032)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Escherichia coli
    • Sequence
      MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH
    • Molecular weight
      21 kDa
    • Amino acids
      1 to 196

Specifications

Our Abpromise guarantee covers the use of ab168032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity is >220 nmoles/min/mg.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    >95% by SDS-PAGE .

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituent: 0.32% Tris HCl

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • FKBP type peptidyl prolyl cis trans isomerase slyD
    • Histidine rich protein
    • PPIase
    • Rotamase
    • WHP
    see all
  • Relevance
    slyD belongs to the FKBP-type PPIase family.Required for lysis of phiX174 infected cells. It binds nickel and zinc with high affinity and 1:1 stoichiometry, copper and cobalt with lower affinity. No binding is detectable for ferrous, ferric, magnesium and calcium ions. The activity of slyD is considerably smaller than the one found in other PPIases with the same substrate. The substrate specificity carried out with 'suc-Ala-Xaa-Pro-Phe-4NA', where Xaa is the AA tested, was found to be Phe > Ala > Leu.
  • Cellular localization
    Cytoplasmic

References

ab168032 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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