Recombinant E. coli Thiol:disulfide interchange protein dsbE (ab117171)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Escherichia coli
    • Sequence
      MRNAEGDDPT NLESALIGKP VPKFRLESLD NPGQFYQADV LTQGKPVLLN VWATWCPTCR AEHQYLNQLS AQGIRVVGMN YKDDRQKAIS WLKELGNPYA LSLFDGDGML GLDLGVYGAP ETFLIDGNGI IRYRHAGDLN PRVWEEEIKP LWEKYSKEAA Q
    • Molecular weight
      18 kDa
    • Amino acids
      26 to 185
    • Additional sequence information
      Recombinant DsbE produced in E.Coli is a single, non-glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18.1 kDa.

Specifications

Our Abpromise guarantee covers the use of ab117171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    ab117171 is purified by proprietary chromatographic techniques.
  • Form
    Liquid
  • Additional notes
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.32% Tris HCl, 0.058% EDTA, 10% Glycerol

General Info

  • Alternative names
    • b2195
    • CcmG
    • Cytochrome c biogenesis protein ccmG
    • dsbE
    • ECK2187
    • JW2183
    • Periplasmic thioredoxin of cytochrome c type biogenesis
    • Thiol:disulfide interchange protein dsbE
    • yejQ
    see all
  • Relevance
    DsbE is a reducing Dsb protein implicated in electron transfer for cytochrome c maturation in the periplasm of Escherichia coli. DsbE is one of 12 proteins required for their assembly in the periplasm. Its postulated function is to reduce disulphide bonds formed between correctly paired cysteine residues in the cytochrome c apoproteins prior to haem attachment by CcmF and CcmH.
  • Cellular localization
    Cell inner membrane; Single-pass membrane protein; Periplasmic side.

References for Recombinant E. coli Thiol:disulfide interchange protein dsbE (ab117171)

ab117171 has not yet been referenced specifically in any publications.

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