The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab117171 is purified by proprietary chromatographic techniques.
Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.50 Constituents: 0.32% Tris HCl, 0.058% EDTA, 10% Glycerol
Cytochrome c biogenesis protein ccmG
Periplasmic thioredoxin of cytochrome c type biogenesis
Thiol:disulfide interchange protein dsbE
RelevanceDsbE is a reducing Dsb protein implicated in electron transfer for cytochrome c maturation in the periplasm of Escherichia coli. DsbE is one of 12 proteins required for their assembly in the periplasm. Its postulated function is to reduce disulphide bonds formed between correctly paired cysteine residues in the cytochrome c apoproteins prior to haem attachment by CcmF and CcmH.