Recombinant E. coli Thiol:disulfide interchange protein dsbE (ab87496)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant E. coli Thiol:disulfide interchange protein dsbE -
Purity
> 95 % SDS-PAGE.
ab87496 is purified using conventional chromatography. -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Escherichia coli -
Sequence
MRNAEGDDPT NLESALIGKP VPKFRLESLD NPGQFYQADV LTQGKPVLLN VWATWCPTCR AEHQYLNQLS AQGIRVVGMN YKDDRQKAIS WLKELGNPYA LSLFDGDGML GLDLGVYGAP ETFLIDGNGI IRYRHAGDLN PRVWEEEIKP LWEKYSKEAA Q -
Amino acids
26 to 185
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Description
Recombinant E. coli Thiol:disulfide interchange protein dsbE
Specifications
Our Abpromise guarantee covers the use of ab87496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.50
Constituents: 0.316% Tris HCl, 0.0584% EDTA, 10% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- b2195
- CcmG
- Cytochrome c biogenesis protein ccmG
see all -
Relevance
DsbE is a reducing Dsb protein implicated in electron transfer for cytochrome c maturation in the periplasm of Escherichia coli. DsbE is one of 12 proteins required for their assembly in the periplasm. Its postulated function is to reduce disulphide bonds formed between correctly paired cysteine residues in the cytochrome c apoproteins prior to haem attachment by CcmF and CcmH. -
Cellular localization
Cell inner membrane; Single-pass membrane protein; Periplasmic side.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab87496 has not yet been referenced specifically in any publications.