Recombinant E. coli Thiol:disulfide interchange protein dsbE (ab87496)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Escherichia coli
    • Sequence
      MRNAEGDDPT NLESALIGKP VPKFRLESLD NPGQFYQADV LTQGKPVLLN VWATWCPTCR AEHQYLNQLS AQGIRVVGMN YKDDRQKAIS WLKELGNPYA LSLFDGDGML GLDLGVYGAP ETFLIDGNGI IRYRHAGDLN PRVWEEEIKP LWEKYSKEAA Q
    • Amino acids
      26 to 185

Specifications

Our Abpromise guarantee covers the use of ab87496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    ab87496 is purified using conventional chromatography.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 2mM EDTA, pH 7.5

General Info

  • Alternative names
    • b2195
    • CcmG
    • Cytochrome c biogenesis protein ccmG
    • dsbE
    • ECK2187
    • JW2183
    • Periplasmic thioredoxin of cytochrome c type biogenesis
    • Thiol:disulfide interchange protein dsbE
    • yejQ
    see all
  • Relevance
    DsbE is a reducing Dsb protein implicated in electron transfer for cytochrome c maturation in the periplasm of Escherichia coli. DsbE is one of 12 proteins required for their assembly in the periplasm. Its postulated function is to reduce disulphide bonds formed between correctly paired cysteine residues in the cytochrome c apoproteins prior to haem attachment by CcmF and CcmH.
  • Cellular localization
    Cell inner membrane; Single-pass membrane protein; Periplasmic side.

Recombinant E. coli Thiol:disulfide interchange protein dsbE images

  • 15% SDS-PAGE analysis of 3µg ab87496.

References for Recombinant E. coli Thiol:disulfide interchange protein dsbE (ab87496)

ab87496 has not yet been referenced specifically in any publications.

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