Recombinant E. coli Thioredoxin / TRX protein (ab115709)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP0AA25
    • SpeciesEscherichia coli
    • Molecular weight12 kDa
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab115709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

    Functional Studies

  • Purity> 95 % SDS-PAGE.
    ab115709 is purified by multi-step chromatography
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.61% Tris, 0.58% Sodium chloride, 0.03% EDTA

General Info

  • Alternative names
    • ADF
    • ATL derived factor
    • ATL-derived factor
    • DKFZp686B1993
    • MGC61975
    • SASP
    • Surface associated sulphydryl protein
    • Surface-associated sulphydryl protein
    • testicular tissue protein Li 199
    • THIO_HUMAN
    • Thioredoxin
    • thioredoxin delta 3
    • TRDX
    • Trx
    • TRX 1
    • TRX1
    • TXN
    • TXN delta 3
    • TXN protein
    • zgc:92903
    see all
  • FunctionParticipates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
    ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
  • Sequence similaritiesBelongs to the thioredoxin family.
    Contains 1 thioredoxin domain.
  • Post-translational
    modifications
    In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
    In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
  • Cellular localizationNucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
  • Information by UniProt

Recombinant E. coli Thioredoxin / TRX protein images

  • Thioredoxin /TRX antibody + Recombinant E. coli Thioredoxin / TRX protein (ab115709) at 0.1 µg
  • SDS-PAGE analysis
    Lane 1: MW markers
    Lane 2: ab115709 at 1 µg
    Lane 3: ab115709 at 2 µg
    Lane 4: ab115709 at 5 µg

References for Recombinant E. coli Thioredoxin / TRX protein (ab115709)

ab115709 has not yet been referenced specifically in any publications.

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