For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
E. coli UNG protein kit (ab215390) catalyzes the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 fewer bases). It is active over a broad pH range with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200 mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites. This product can be used for: Glycosylase mediated single nucleotide polymorphism detection (GMPD). Site-directed mutagenesis. As a probe for protein-DNA interaction studies. Rapid and efficient cloning of PCR products. Elimination carry-over contamination in PCR. Inactivated by heating at 95°C for 10min. Enzyme activity is partially restored at temperatures lower than 55°C.
1 Unit is defined as the amount of UDG enzyme that catalyzes release of 60 pmol of Uracil per minute from double-stranded, uracil-containing DNA in a total reaction volume of 50 µL in 30 minutes at 37°C in 1X UNG Reaction Buffer (200 mM Tris-HCl (pH 8.0 at 25°C), 10 mM DTT and 10 mM EDTA) with 1 Unit of UNG and 0.2 µg [3H]-Uracil DNA (104- 105 cpm/µg).
The specific activity is 5 U/µL.
The UNG protein was purified by standard chromatographic techniques.
|10X UNG Reaction Buffer||1 x 2ml|
|UNG Enzyme||1 vial|
|UNG Storage Buffer||1 x 2ml|
Our Abpromise guarantee covers the use of ab215390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab215390 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"