Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SequenceMFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
    • Molecular weight17 kDa
    • Additional sequence informationThis product is the mature full length protein from aa 16 to 155. The propeptide is not included.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab9592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level< 0.100 Eu/µg
  • FormLyophilised
  • Additional notesThe ED50, determined by the dose-dependent stimulation of the proliferation of thymidine uptake by BaF3 cells expressing FGF receptors, is < 10.0 ng/ml, corresponding to a specific activity of > 1.0 x 105 units/mg.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 5mM Sodium phosphate, 100mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Acidic fibroblast growth factor
    • aFGF
    • Beta endothelial cell growth factor
    • Beta-endothelial cell growth factor
    • ECGF
    • ECGF beta
    • ECGF-beta
    • ECGFA
    • ECGFB
    • Endothelial Cell Growth Factor alpha
    • Endothelial Cell Growth Factor beta
    • FGF 1
    • FGF alpha
    • Fgf1
    • FGF1_HUMAN
    • FGFA
    • Fibroblast growth factor 1
    • Fibroblast Growth Factor 1 Acidic
    • GLIO703
    • HBGF 1
    • HBGF-1
    • HBGF1
    • Heparin binding growth factor 1
    • Heparin binding growth factor 1 precursor
    • Heparin-binding growth factor 1
    see all
  • FunctionThe heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
  • Sequence similaritiesBelongs to the heparin-binding growth factors family.
  • Cellular localizationSecreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
  • Information by UniProt

References for Recombinant FGF1 protein (ab9592)

This product has been referenced in:
  • Sletten T  et al. Nucleolin regulates phosphorylation and nuclear export of fibroblast growth factor 1 (FGF1). PLoS One 9:e90687 (2014). Read more (PubMed: 24595027) »

See 1 Publication for this product

Product Wall

Application Cellular Activation
The product containing full-length FGF1 was fully active in fibroblast proliferation studies, as compared to FGF1 produced in our lab. We have also performed very good binding studies using the protein, including pulldown and SPR techniques.
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Submitted Sep 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"