Recombinant Gliadin protein (ab124981)

Overview

  • Product nameRecombinant Gliadin protein
  • Protein lengthProtein fragment

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • Molecular weight19 kDa including tags

Specifications

Our Abpromise guarantee covers the use of ab124981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.
    Purity is greater than 94.0% as determined by SDS-PAGE.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.48% HEPES, 20% Glycerol, 1.17% Sodium chloride

General Info

  • Alternative names
    • prolamin
  • RelevanceCeliac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The alcohol soluble proteins (prolamins) from wheat, rye, barley and oats produce the harmful effect of coeliac disease or gluten sensitive enteropathy in humans by causing characteristic changes in the intestinal mucosa. Patients so affected must avoid eating these grains and replace them with rice, corn, potatoes, etc. Many gluten-free foods are produced industrially, thus several immunoassays have been developed for determination of gliadin in supposedly gluten-free foods.
  • Cellular localizationCytoplasmic; major seed storage protein in wheat.

References for Recombinant Gliadin protein (ab124981)

ab124981 has not yet been referenced specifically in any publications.

Product Wall



We received the following information from the lab regarding ab124981 :

The protein sequence was not designed in respect to the question whether the fragment shows sequence homologies with alpha/beta gliadin.

We cannot exc...

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No, unfortunately we do not have an alpha/beta gliadin, just this gamma gliadin fragment. I have not heard back from the laboratory that supplies the antibody ab50602 to us regarding whether reactivity with gamma gliadin has be assessed. I think it is ...

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I am looking into the possibility of ab50602 cross-reacting with the gamma gliadin fragment ab124981. The antibody is raised against alpha/beta gliadin, which according to my alignment, does not share much sequence with gamma gliadin. I have asked the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"