The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. Purified by chromatography under denaturing conditions.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C.
ReconstitutionReconstitute with PBS or 8M urea to give 0.5mg/ml concentration. After reconstitution in PBS aliquot and store at -20°C. Avoid freeze / thaw cycles. Proteins reconstituted in 8M urea stored at 4°C.
RelevanceDobrava virus (DOBV) which can be carried by the yellow-necked field mouse Apodemus flavicollis is one of the hantaviruses that can cause severe hemorrhagic fever with renal syndrome in humans. For several hantaviruses, the nucleocapsid (N) protein has proven to be very immunogenic in humans and rodents and even can protect rodents against a virus challenge.
Recombinant Hantavirus Dobrava, strain Slovenia, nucleocapsid protein images