Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus

Specifications

Our Abpromise guarantee covers the use of ab42631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Activity: 110 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 uM Biomol substrate (Catalog number KI177), and 1 ng/ul HDAC3/NcoR2. Incubation condition: 30 min at 37ºC.
  • Applications

    Inhibition Assay

  • Purity
    > 90 % SDS-PAGE.

  • Form
    Liquid
  • Additional notes
    Co-expressed in a Baculovirus infected Sf9 cell expression system.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 50mM Tris HCl, 20mM Glutathione, 138mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • CTG26
    • HD 3
    • HD3
    • HDAC 3
    • HDAC3
    • HDAC3_HUMAN
    • Histone deacetylase 3
    • NCOR 2
    • Nuclear receptor co repressor 2
    • RPD3
    • RPD3-2
    • SMAP45
    • SMRTE
    • SMRTE tau
    • TNRC14
    • TRAC1
    see all
  • Function
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications
    Sumoylated in vitro.
  • Cellular localization
    Nucleus.
  • Information by UniProt

References

This product has been referenced in:
  • Demmerle J  et al. The nuclear envelope protein emerin binds directly to histone deacetylase 3 (HDAC3) and activates HDAC3 activity. J Biol Chem : (2012). Read more (PubMed: 22570481) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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