Recombinant Hepatitis C Virus Core 2a protein (ab123482)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ99IB8
    • Molecular weight29 kDa
    • Amino acids1 to 271
    • TagsHis tag C-Terminus
    • Additional sequence informationThe NS4A co-factor is fused to the N-terminus of the NS3 protease domain.

Specifications

Our Abpromise guarantee covers the use of ab123482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityOne unit of protease hydrolyzes 1 picomole of Ac-Asp-Glu-Dap(QXL™520)- Glu-Glu-Abu-COO-Ala -Ser-Cys(5-FAMsp)-NH2 per minute at pH 7.5 at 25° C.
    ab123482 is in active form and the pre-activation by pep4A or pep4AK is not necessary.
  • Applications

    Functional Studies

  • FormLiquid
  • Additional notes

    NS3 protease of hepatitis C virus (HCV), located on the N-terminal domain of HCV NS3 is responsible for the cleavage at the NS3/NS4A, NS4A/NS4B, NS4B/NS5A, and NS5A/NS5B sites of the nonstructural protein. The HCV NS3 is a chymotrypsin-like serine protease. It requires a cofactor, a 54 amino acid NS4 protein, to reach its optimal activity. The X-ray crystal structure studies show that NS3 forms a tight non-covalent complex with NS4.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.75% Potassium chloride, 0.02% DTT, 0.32% Tris HCl, 0.006% EDTA, 20% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Genome polyprotein
    • HCV 2a
    • HCV core 2a
    • HCV Core genotype 2a
    • HCV2a
    • Hepatitis C Virus Core genotype 2a
    see all
  • RelevanceHCV (Hepatitis C Virus) viral core protein forms the internal viral coat that encapsidates the genomic RNA and is enveloped in a host cell-derived lipid membrane. The hepatitis C virus (HCV) core protein represents the first 177 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. The N terminus of the core protein is involved in transcriptional repression. There are over 20 different subtypes of Hepatitis C Virus; the preponderance and distribution of HCV genotypes varies globally. HCV Genotype information is important because of the role it plays in predicting HCV medical treatment response and treatment duration. Sustained cure rates (sustained viral response) of 75% or better occur in people with genotypes HCV 2 and 3 in 24 weeks of treatment. Genotypes 1a and 1b, the most prevalent worldwide, have the poorest rates of response to the present treatment regimen, which is a combination of pegylated alfa interferon 2b with ribavirin. HCV core protein is among the most conserved proteins in HCV and is known to induce sensitization of cytotoxic T lymphocytes (CTL). Therefore, it is a prime candidate for a component of a potential HCV vaccine.

References for Recombinant Hepatitis C Virus Core 2a protein (ab123482)

ab123482 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"