The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityOne unit of protease hydrolyzes 1 picomole of Ac-Asp-Glu-Dap(QXL™520)-
Glu-Glu-Abu-COO-Ala -Ser-Cys(5-FAMsp)-NH2) per minute at pH 7.5 at 25° C.
ab123483 is in active form and the pre-activation by pep4A or pep4AK is not necessary.
NS3 protease of hepatitis C virus (HCV), located on the N-terminal domain of HCV NS3, is responsible for the cleavage at the NS3/NS4A, NS4A/NS4B, NS4B/NS5A, and NS5A/NS5B sites of the nonstructural protein. The HCV NS3 is a chymotrypsin-like serine protease. It requires a cofactor, a 54 amino acid NS4 protein, to reach its optimal activity. The X-ray crystal structure studies show that NS3 forms a tight non-covalent complex with NS4. The NS3/4A protease is essential for viral replication and the formation of infectious viral particles.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Serine protease/NTPase/helicase NS3
RelevanceThe nonstructural protein NS3 of the hepatitis C virus (HCV) is indispensable for virus replication and a multifunctional enzyme that contains three catalytic activities such as serine protease, helicase, and NTPase. The N-terminal domain of the protein contains protease activity and the C-terminal domain contains nucleotide triphosphatase and RNA helicase activity. It has been shown that NS2/3 cleavage is mediated by NS2-3 protease, whereas NS3 serine protease is responsible for the other four cleavage sites of the nonstructural (NS) region.
Immunoblot analysis on serum samples from 90 patients with chronic hepatitis C virus infection revealed four putative immunogenic regions within the NS3 protein of the virus: E (around amino acids 1250/1251), A (within amino acids 1250-1334), A/B (around amino acids 1323 and 1334), and B/C (around amino acids 1407 and 1412). Among them, region E was most immunodominant, and region A was recognized much less frequently by patients with cirrhosis than by those with chronic hepatitis.
Cellular localizationHost endoplasmic reticulum membrane; Peripheral membrane protein. Note: NS3 is associated to the ER membrane through its binding to NS4A.
References for Recombinant Hepatitis C virus Genotype 1a NS3 protein (ab123483)
has not yet been referenced specifically in any publications.
Publishing research using ab123483? Please let us know so that we can cite the reference in this datasheet.
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