Recombinant Hepatitis C Virus Hepatitis Virus Genotype 1b NS3 protein (ab67962)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Hepatitis C virus
    • Sequence
      Starts: MRDSDSQTFQ Ends: DSVIDCNTCVT
    • Molecular weight
      26 kDa
    • Additional sequence information
      The E.coli derived 26.2 kDa recombinant protein contains the HCV NS3 c33c immunodominant regions. The 252 amino acid protein is fused to 6xHis tag.

Specifications

Our Abpromise guarantee covers the use of ab67962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • Purity
    > 95 % SDS-PAGE.
    ab67962 is purified by a proprietary chromatographic technique.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C.

    Preservative: None
    Constituents: 8mM DTT, 50mM Sodium phosphate, pH 8.3

General Info

  • Alternative names
    • Hepatitis Virus Genotype 1b NS3
  • Relevance
    HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein.

References for Recombinant Hepatitis C Virus Hepatitis Virus Genotype 1b NS3 protein (ab67962)

ab67962 has not yet been referenced specifically in any publications.

Publishing research using ab67962? Please let us know so that we can cite the reference in this datasheet.

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