Recombinant Hepatitis C Virus Hepatitis Virus Genotype 1b NS3 protein (ab67962)
- Datasheet
- Specific References
- Protocols
Overview
- Product nameRecombinant Hepatitis C Virus Hepatitis Virus Genotype 1b NS3 protein
See all Hepatitis Virus Genotype 1b NS3 proteins and peptides - Protein lengthProtein fragment
Description
- NatureRecombinant
- SourceEscherichia coli
- Amino Acid Sequence
- SpeciesHepatitis C virus
- SequenceStarts: MRDSDSQTFQ Ends: DSVIDCNTCVT
- Molecular weight26 kDa
- Additional sequence informationThe E.coli derived 26.2 kDa recombinant protein contains the HCV NS3 c33c immunodominant regions. The 252 amino acid protein is fused to 6xHis tag.
Associated products
- Related Products
Specifications
Our Abpromise guarantee covers the use of ab67962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Applications
Western blot
ELISA
- Purity> 95
% SDS-PAGE.
ab67962 is purified by a proprietary chromatographic technique. - FormLiquid
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Concentration information loading...
Preparation and Storage
- Stability and Storage
Shipped at 4°C. Store at -20°C.
Preservative: None
Constituents: 8mM DTT, 50mM Sodium phosphate, pH 8.3
General Info
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Alternative names
- Hepatitis Virus Genotype 1b NS3
- RelevanceHCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein.
References for Recombinant Hepatitis C Virus Hepatitis Virus Genotype 1b NS3 protein (ab67962)
ab67962 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"