Recombinant Hepatitis C Virus NS3 (mutated A156 T) protein (ab123463)



  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • Amino acids1 to 217
    • Modificationsmutated A156


Our Abpromise guarantee covers the use of ab123463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity1 unit of protease hydrolyzes 1 picomole of Ac-Asp-Glu-Dap(QXL™520)-Glu- Glu-Abu-COO-Ala -Ser-Cys(5-FAMsp)-NH2 (AnaSpec Cat# 71145) per minute at pH 7.5 at 25° C.
    Note: pre-activation by pep4A or pep4AK is not necessary
  • Applications

    Functional Studies

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.75% Potassium chloride, 0.02% DTT, 0.32% Tris HCl, 0.006% EDTA, 20% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Hepatitis C Virus NS3
    • NS3
    • NS3P
    • p70
    • Serine protease/NTPase/helicase
    see all
  • RelevanceHCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
  • Cellular localizationEndoplasmic reticulum membrane

References for Recombinant Hepatitis C Virus NS3 (mutated A156 T) protein (ab123463)

ab123463 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

The product ab84346 has not been tested for helicase activity but I have asked our source for the protein to confirm. I will forward what I receive from them.

We do not have a full-length NS3. There is...

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