The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activity1 unit of protease hydrolyzes 1 picomole of Ac-Asp-Glu-Dap(QXL™520)-Glu-
Glu-Abu-COO-Ala -Ser-Cys(5-FAMsp)-NH2 per minute at pH 7.5 at 25° C. ab123539 is in the active form and the preactivation by pep4A or pep4AK is not necessary.
ab123539 is derived from its wild type (NCBIAccession: CAB46913) with a substitution of Thr54 with Ala. The mutations, T54A, conferred low to moderate levels of resistance (<20-fold) to NS3 protease inhibitor SCH503034.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
References for Recombinant Hepatitis C Virus NS3 + NS4 (mutated T54 A) protein (ab123539)
has not yet been referenced specifically in any publications.
Publishing research using ab123539? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"