Recombinant Hepatitis C Virus NS3 protein (ab84346)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% Affinity purified
- Tags: His tag N-Terminus
- Suitable for: Functional Studies
Description
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Product name
Recombinant Hepatitis C Virus NS3 protein
See all Hepatitis C Virus NS3 proteins and peptides -
Biological activity
1 unit equals 1 nanogram of purified protein. -
Purity
> 95 % Affinity purified.
Recombinant ab84346 was expressed in a E.coli system and purified by an affinity column in combination with FPLC chromatography. The purified protein is greater than 95% homogeneous and contains no detectable DNase and RNase activity. -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Predicted molecular weight
23 kDa -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab84346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
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Form
Liquid -
Additional notes
1 unit equals 1 nanogram of purified protein. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.9
Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- Hepatitis C Virus NS3
- NS3
- NS3P
see all -
Relevance
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa. -
Cellular localization
Endoplasmic reticulum membrane
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab84346 has not yet been referenced specifically in any publications.