The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
One unit of protease hydrolyzes 1 picomole of Ac-Asp-Glu-Dap(QXL™520)-
Glu-Glu-Abu-COO-Ala -Ser-Cys(5-FAMsp)-NH2 per minute at pH 7.5 at 25° C. ab123540 is in active form and the pre-activation by pep4A or pep4AK is not necessary.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Hepatitis Virus Genotype 1b NS3
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein.
References for Recombinant Hepatitis Virus Genotype 1b NS3 protein (ab123540)
has not yet been referenced specifically in any publications.
Publishing research using ab123540? Please let us know so that we can cite the reference in this datasheet.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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