Recombinant HIV-1 gp120 (Du172.17 protein (ab167715)

Overview

  • Product name
    Recombinant HIV-1 gp120 (Du172.17 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Sequence
      The predicted N-terminus is Thr27.
    • Molecular weight
      54 kDa including tags
    • Amino acids
      27 to 498
    • Tags
      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab167715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Endotoxin level
    < 1.000 Eu/µg
  • Purity
    > 97 % SDS-PAGE.

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: 99% PBS
    Note: Normally Mannitol or Trehalose are added as protectants before lyophilization

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in 10µl sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names
    • gp120
    • gp120 glycoprotein
    • Human Immunodeficiency Virus 1
    • SU
    • Surface protein
    see all
  • Relevance
    HIV1 is equipped with the envelope gp160 glycoprotein for interaction with Langerhans cells (LCs) and dendritic cells (DCs), the members of the innate immune system, which confront the virus at the portal of virus entry in the human body. These cells are equipped with receptors by which they bind and endocytose the virus. The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response.
  • Cellular localization
    Cell Membrane

Images

  • SDS-PAGE of reduced ab167715 stained overnight with Coomassie Blue.

References

ab167715 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
ELISA
Procedure for detection with HRP conjugated antibody:
Wells A1 and A2 were coated with 100µl/well of 1µg/mL of gp120 diluted with a carbonate coating buffer (pH 9.80). 100µl/well of empty coating buffer was added to wells B1, B2, C1 and C2. The plate was covered with Parafilm and incubated overnight at 4℃. All wells were washed with 180µl/well of PBST 0.05% a total of 5 times allowing ≈30 seconds of soak time, and then slapped dry. All wells were blocked with 200µl/well of 1X Assay Diluent for 2hr at RT at 40RPM. Washing procedure was repeated. The HRP conjugated antibody (ab106578) (self-conjugated according to protocol for Lightning Link HRP Conjugation Kit) was added at 100µl/well at 1µg/mL and incubated for 2hrs at RT at 40RPM. Washing procedure was repeated with a total of 7 wash cycles. 100µl/well of TMB was added, and after 15 min 50µl/well of stop solution (2N H2SO4) was added. The plate was read at 450nm (Biotek instrument).

Procedure for detection with Biotin conjugated antibody:
Wells A1 and A2 were coated with 100µl/well of 1µg/mL of gp120 diluted with a carbonate coating buffer (pH 9.80). 100µl/well of empty coating buffer was added to wells B1, B2, C1 and C2. The plate was covered with Parafilm and incubated overnight at 4℃. All wells were washed with 180µl/well of PBST 0.05% a total of 5 times allowing ≈30 seconds of soak time, and then slapped dry. All wells were blocked with 200µl/well of 1X Assay Diluent for 2hr at RT at 40RPM. Washing procedure was repeated. The Biotin conjugated antibody (ab106578) (self-conjugated according to protocol for Lightning Link Rapid Biotin Conjugation Kit) was added at 100µl/well at 1µg/mL (diluted with 1X Assay Diluent) and incubated for 2hrs at RT at 40RPM. Washing procedure was repeated. 100µl/well of Avidin-HRP was added at a dilution factor of 1:250 (diluted with 1X Assay Diluent). Washing procedure was repeated with a total of 7 wash cycles. 100µl/well of TMB was added, and after 15 min 50µl/well of stop solution (2N H2SO4) was added. The plate was read at 450nm (Biotek instrument).

Sample preparation of recombinant gp120:
100µl of Type 1 ultrapure water (Milli-Q) and 100µl of 0.2% BSA were added to the recombinant protein. The vial was gently shaken to ensure no foam forms. It was then incubated at RT for 1hr with gentle shaking every 15 minutes. The reconstituted protein was then aliquoted and stored at -20℃ in protein low bind tubes.
Username

Kami Krista

Verified customer

Submitted Sep 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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