Recombinant HIV tat (mutated C22) protein (ab83352)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • Modificationsmutated C22

Specifications

Our Abpromise guarantee covers the use of ab83352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityReacts with anti-Tat antibodies from human, monkey, rabbit and mouse serum.
  • Applications

    Blocking

    Functional Studies

    Western blot

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.

  • FormLyophilised
  • Additional notesReacts with anti-Tat antibodies from human, monkey, rabbit and mouse serum.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C.

    Preservative: None
    Constituents: 20mM NaH2PO4/ Na2HPO4, 2.5% Glycerol, 1.2M Sodium chloride, 50mM Mannitol, 1mM DTT, pH 7.5

  • ReconstitutionThe protein should be reconstituted in apirogenic and sterile dH2O. The reconstituted solution has to be used immediately, since it is not stable in liquid form.

General Info

  • Alternative names
    • p14
    • Tat
    • Tat protein
    • Transactivating regulatory protein
  • RelevanceThe transcriptional transactivator (Tat) is a key regulatory protein of HIV. It is expressed early after the virus integrates into the cell, and stimulates the elongation of RNA polymerase II. It binds onto a sequence known as the TAR, or transactivator response element, located at the end of the HIV genetic chain. There, the tat protein helps assemble new copies of HIV. The tat protein-TAR complex speeds up the rate of viral reproduction by about a thousand times. If it is not present, the transcription process frequently stops short, and few functional HIV particles are produced. Tat is an important potential target for antiretrovirals and vaccine development.

References for Recombinant HIV tat (mutated C22) protein (ab83352)

ab83352 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"