Recombinant HIV tat protein (ab109949)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Amino acids
      1 to 101

Specifications

Our Abpromise guarantee covers the use of ab109949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity
    > 98 % SDS-PAGE.

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 0.1% Glycerol

  • Reconstitution
    The protein should be reconstituted in apirogenic sterile water or PBS buffer. The reconstituted solution has to be used immediately. (Avoid repeated freeze/thaw cycles).

General Info

  • Alternative names
    • p14
    • Tat
    • Tat protein
    • Transactivating regulatory protein
  • Relevance
    The transcriptional transactivator (Tat) is a key regulatory protein of HIV. It is expressed early after the virus integrates into the cell, and stimulates the elongation of RNA polymerase II. It binds onto a sequence known as the TAR, or transactivator response element, located at the end of the HIV genetic chain. There, the tat protein helps assemble new copies of HIV. The tat protein-TAR complex speeds up the rate of viral reproduction by about a thousand times. If it is not present, the transcription process frequently stops short, and few functional HIV particles are produced. Tat is an important potential target for antiretrovirals and vaccine development.

References

ab109949 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us and your interest in our products.

We have as yet not used the HIV tat protein (ab109949) in ELISA so I cannot provide a protocol for how this application may be performed. This does not mean that it would not work...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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