Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ9WPY4
    • Molecular weight57 kDa
    • Amino acids1 to 508

Associated products

Specifications

Our Abpromise guarantee covers the use of ab109969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.

  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C.

    Preservative: None
    Constituents: 1% Glycerol

  • ReconstitutionThe protein should be reconstituted in apirogenic sterile water or PBS buffer. The reconstituted solution has to be used immediately. (Avoid repeated freeze/thaw cycles).

General Info

  • Alternative names
    • Capsid
    • Gag polyprotein
    • Matrix
    • Nucleocapsid
    • p1
    • p2
    • p6
    • Pr55(Gag)
    • Pr55Gag
    see all
  • RelevanceThe Gag protein is the major structural protein required for virus assembly. It is synthesized as a polyprotein in the cytosol of an infected cell, and contains four functional segments; Matrix (MA), Capsid (CA), Nucleocapsid (NC), and p6. The NC region is flanked by two "spacer" segments, denoted SP1 and SP2. The polyprotein is all alpha helical, except the NC region, which is composed of two RNA interacting zinc knuckle domains. Gag is often referred to as an "assembly machine" because expression of Gag alone is sufficient to produce budding virus-like particles (VLP's), due to multimerization of roughly 2000 Gag molecules per virion. Gag is cleaved by the protease at multiple sites. The GAG proteins play important roles throughout the viral life-cycle, including the assembly and release of viral particles, their subsequent maturation into infectious virions, and during the events occurring between the release of capsids into newly infected cells and the integration of proviral DNA. During the early steps of the viral life cycle, viral proteins, especially capsid (CA), are in intimate contact with the intracellular environment. Considerable evidence supports the idea that interactions between host cellular proteins and the viral capsid are important for events occurring early in infection, such as the transport of the preintegration complex, uncoating of the capsid, nuclear entry, and integration Gag capsid (CA) protein can markedly reduce viral fitness, and interactions of CA with host proteins such as cyclophilin A (CypA) and TRIM5alpha can have important effects on viral infectivity.

Recombinant HIV1 Gag protein images

  • ab109969 is >90% pure estimated by SDS-PAGE.

References for Recombinant HIV1 Gag protein (ab109969)

ab109969 has not yet been referenced specifically in any publications.

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