Recombinant HIV1 gp120 + gp41 protein (ab49054)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: ELISA, WB
Description
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Product name
Recombinant HIV1 gp120 + gp41 protein
See all HIV1 gp120 + gp41 proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino acids
466 to 753 -
Additional sequence information
a.a 466 to a.a 753 of the HIV-1 env region. Fusions: β-galactosidase (114 kDa) fused at the N-terminus.
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Specifications
Our Abpromise guarantee covers the use of ab49054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
Western blot
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Form
Liquid -
Additional notes
This protein can be used to detect all HIV-1 and HIV-type O infected individuals responding to envelope proteins.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7.2
Constituents: 0.106% Sodium carbonate, 0.1092% Beta mercaptoethanol, 0.05% Tween, 0.292% EDTA
General Info
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Alternative names
- Glycoprotein 120
- Glycoprotein 41
- HIV1 gp120
see all -
Relevance
gp41/120 is the major HIV protein associated with the HIV envelope. It functions as the viral antireceptor or attachment protein. gp41 (or TM) traverses the envelope, whereas gp120 is present on the outer surface and is noncovalently attached to gp41. The precursor of gp120/41 (gp160) is synthesized in the endoplasmic reticulum and is transported via the golgi body to the cell surface. Upon activation of the envelope glycoprotein (gp120/41) by cellular receptors, gp41 undergoes conformational changes that mediate fusion of the viral and cellular membranes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab49054 has not yet been referenced specifically in any publications.