Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli

Associated products

Specifications

Our Abpromise guarantee covers the use of ab63996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 90 % SDS-PAGE.
    Highly purified by several steps of chromatography.
  • Form
    Liquid
  • Additional notes
    ab63996 has been derived from infectious plasmid clone pNL4-3 and belongs to Group B of HIV-1.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 50mM Sodium chloride, 20mM Tris HCl, 10mM Beta mercaptoethanol, pH 7.5

General Info

  • Alternative names
    • C terminal core protein
    • F protein
    • Nef
    • Negative factor
    • p27
    see all
  • Relevance
    Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.

Images

  • Polyacrylamide gel electrophoresis of ab63996.

References

ab63996 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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