Recombinant HIV1 Nef protein (Beta-Galactosidase) (ab49060)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Additional sequence information
      Fused with β-galactosidase at the N-terminus.

Specifications

Our Abpromise guarantee covers the use of ab49060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Reacts strongly with human HIV positive serum.
  • Applications

    ELISA

    Western blot

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Liquid
  • Additional notes
    Reacts strongly with human HIV positive serum.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 0.014M Beta mercaptoethanol, 0.01M Sodium carbonate, 0.01M EDTA

General Info

  • Alternative names
    • C terminal core protein
    • F protein
    • Nef
    • Negative factor
    • p27
    see all
  • Relevance
    Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.

References

ab49060 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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