Recombinant HIV1 p55 protein (ab63995)

Overview

  • Product name
    Recombinant HIV1 p55 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence

Specifications

Our Abpromise guarantee covers the use of ab63995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 90 % SDS-PAGE.
    Purified by several steps of chromatography
  • Form
    Liquid
  • Additional notes

    ab63995 has been derived from infectious plasmid clone pNL4-3 and belongs to Group B of HIV-1.

    Active as HIV-1 protease substrate

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 50mM Sodium chloride, 20mM Tris HCl, 10mM Beta mercaptoethanol, pH 7.5

General Info

  • Alternative names
    • gag
    • Gag polyprotein
    • Pr55Gag
  • Relevance
    HIV1 (gag) p55 is a precursor protein of several proteins that form the core structure of AIDS virus, indispensable to its reproduction. This protein is digested by HIV1 protease, first into intermediate products p41 and p15. Then p41 is digested into matrix protein p17 and capsid protein p24. Protein p15 is further digested into nucleocapsid protein p7 and to p6 and p1; of unknown function. Nine genes are encoded within the HIV1 RNA genome. Three of these genes, gag, pol, and env, contain information needed to make the structural proteins for new virus particles.

Images

  • SDS-PAGE analysis of Recombinant HIV1 p55 protein. As the substrate, Recombinant HIV1 p55 protein (1 μg, ab63995) was used in 20 μl reaction volume. The reaction was carried by incubating at 37℃ for 3 h and stopped by adding SDS-PAGE sample buffer. Lane 1: no protease,
    Lane 2: 0.16 pg.
    Lane 3: 1.6 pg.
    Lane 4: 16 pg
    Lane 5: 0.16 μg
    Lane 6: 1.6 μg protease.
    Note that two degradation bands are observed in the preparation of p55 substrate. In lane 4, p25 band is visible and in lane 5, p13 band is visible.

  • Polyacrylamide gel electrophoresis of ab63995.
    The arrows show degradation products.

References

ab63995 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

HIV-1 p55 protein was not lyophilized during purification step. I hope this is helpful. Please contact me again if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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