Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SequencePQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGI GGFIKVRQYDQILIEICGHK
    • Amino acids1 to 99

Associated products

Specifications

Our Abpromise guarantee covers the use of ab84117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    One unit of HIV Protease hydrolyzes 1 picomole of a peptide (SQNYPIVQ) per minute at pH 4.7 at 25oC. 20-200ng is sufficient for an in vitro protease assay. HIV Protease can be applied in in vitro assay development and screening of protease inhibitors.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity> 95 % SDS-PAGE.
    Purified by an affinity chromatography in combination with FPLC chromatography
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, 500mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • HIV-1 protease

References for Recombinant HIV1 Protease protein (ab84117)

This product has been referenced in:
  • Boso G  et al. The nature of the N-terminal amino acid residue of HIV-1 RNase H is critical for the stability of reverse transcriptase in viral particles. J Virol 89:1286-97 (2015). Read more (PubMed: 25392207) »

See 1 Publication for this product

Product Wall

Thank you for contacting us yesterday and you interest in our products.

I have had a look into your query relating to whether the HIV protease proteins (ab113857, ab124313, ab124858 and ab84117) are all active. The title you have noticed wher...

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Thank you for your patience. I apologize for the delay in getting back to you. I just received the reply from the supplying lab today. "For our experiments measuring the enzymatic activity, we have used both i) 100 mM MES (pH6), 200 mM NaCl, 0....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"