The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityab83353 reacts with anti-Tat antibodies from human, monkey, rabbit and mouse serum.
Additional notesab83353 reacts with anti-Tat antibodies from human, monkey, rabbit and mouse serum.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C.
Constituents: 0.1% Glycerol
ReconstitutionThe protein should be reconstituted in apirogenic sterile water or PBS buffer. The reconstituted solution has to be used immediately.
Transactivating regulatory protein
RelevanceHIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
Cellular localizationNuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
References for Recombinant HIV1 tat protein (ab83353)
This product has been referenced in:
Qu J et al. The Tat protein of human immunodeficiency virus-1 enhances hepatitis C virus replication through interferon gamma-inducible protein-10. BMC Immunol13:15 (2012).
Read more (PubMed: 22471703) »