The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Cytotoxicity can be detected in Human neutrophils when used in combination with Hlg B in a concentration range of 0.03-100 nM.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 90% PBS, 10% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Gamma-hemolysin component C
Toxin that seems to act by forming pores in the membrane of the cell. Has a hemolytic and a leucotoxic activity.
Western blot - Active HlgC full length protein (ab190123)
All lanes : Rabbit polyclonal anti HIgC at 0.5 µg/ml
Lane 1 :Recombinant HlgC protein (His tag) (ab190123) at 0.4 µg Lane 2 :Recombinant HlgC protein (His tag) (ab190123) at 0.2 µg Lane 3 :Recombinant HlgC protein (His tag) (ab190123) at 0.1 µg Lane 4 :Recombinant HlgC protein (His tag) (ab190123) at 0.05 µg Lane 5 :Recombinant HlgC protein (His tag) (ab190123) at 0.025 µg Lane 6 :Recombinant HlgC protein (His tag) (ab190123) at 0.0125 µg
Secondary All lanes : anti-rabbit IgG-alkaline phosphatase (AP) conjugate, followed by AP substrate.
SDS-PAGE - Active HlgC full length protein (ab190123)
SDS-PAGE and stain demonstrating 1 μg and 5 μg (lanes 1 and 2, respectively) of protein ab190123 under denaturing and reducing conditions.
Functional Studies - Active HlgC full length protein (ab190123)
Toxin Functionality: Human promyleocytic leukemia cell line HL60 was differentiated into neutrophils by treatment with DMSO. Neutrophils were incubated with serial dilutions of ab190123 and HlgC at equimolar concentration for 48 hours at 37°C with 5% CO2 and 95% humidity. Cellular viability was determined by adding XTT and incubation for additional 16 hours. OD’s were determined in the supernatants at 470nm. EC50 was found to be 8.92 nM of each toxin subunit.
has not yet been referenced specifically in any publications.
Publishing research using ab190123? Please let us know so that we can cite the reference in this datasheet.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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