Recombinant HSF1 protein (ab115715)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ00613
    • Molecular weight70 kDa including tags
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab115715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity>= 90 % SDS-PAGE.
    ab115715 is purified by multi-step chromatography
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Constituents: 5% Glycerol, 99% Tris buffered saline

General Info

  • Alternative names
    • Heat shock factor 1
    • Heat shock factor protein 1
    • Heat shock transcription factor 1
    • HSF 1
    • hsf1
    • HSF1_HUMAN
    • HSTF 1
    • HSTF1
    see all
  • FunctionDNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
  • Sequence similaritiesBelongs to the HSF family.
  • Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    modifications
    Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
    Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
  • Information by UniProt

Recombinant HSF1 protein images

  • Polyclonal HSF1 antibody + Recombinant HSF1 protein (ab115715) at 0.01 µg
  • SDS-PAGE analysis
    Lane 1: MW marker
    Lane 2: ab115715 at 2 mg

References for Recombinant HSF1 protein (ab115715)

ab115715 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

I have heard back from the lab, and they have confirmed that ab117515 is phosphorylated in vitro to produce ab115508.
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