Recombinant Human 14-3-3 eta protein (ab116442)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMGDREQLLQRARLAEQ AERYDDMASAMKAVTELNEPLSNEDRNLLSVAYKNVVGARRSSWRVISSI EQKTMADGNEKKLEKVKAYREKIEKELETVCNDVLSLLDKFLIKNCNDFQ YESKVFYLKMKGDYYRYLAEVASGEKKNSVVEASEAAYKEAFEISKEQMQ PTHPIRLGLALNFSVFYYEIQNAPEQACLLAKQAFDDAIAELDTLNEDSY KDSTLIMQLLRDNLTLWTSDQQDEEAGEGN
    • Molecular weight
      32 kDa including tags
    • Amino acids
      1 to 246
    • Tags
      His tag N-Terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab116442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF-TOF
  • Purity
    > 80 % Purified via His tag.
    ab116442 was purified by Ni2+-NTA agarose
  • Form
    Liquid
  • Additional notes
    ab116442 was alkylated, digested with trypsin and the mass of the resultant peptides determined by MADLI-TOF/TOF. The peptides obtained gave 67% sequence coverage of 14-3-3 eta. Theoretical pI: 5.3
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.6% HEPES, 0.02% DTT, 50% Glycerol, 0.012% Benzamidine, 0.003% PMSF

General Info

  • Alternative names
    • 14 3 3 protein eta
    • 14-3-3 eta
    • 14-3-3 protein eta
    • 1433F_HUMAN
    • Brain protein 14-3-3, eta isoform
    • HGNC:12853
    • Protein AS1
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, eta isoform
    • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide
    • YWHA 1
    • YWHA1
    • Ywhah
    see all
  • Function
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • Tissue specificity
    Expressed mainly in the brain and present in other tissues albeit at lower levels.
  • Sequence similarities
    Belongs to the 14-3-3 family.
  • Information by UniProt

Images

  • 12 % SDS-PAGE gel showing ab116442.

References

ab116442 has not yet been referenced specifically in any publications.

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