Recombinant Human 14-3-3 gamma protein (ab116443)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMVDREQLVQKARLAEQ AERYDDMAAAMKNVTELNEPLSNEERNLLSVAYKNVVGARRSSWRVISSI EQKTSADGNEKKIEMVRAYREKIEKELEAVCQDVLSLLDNYLIKNCSETQ YESKVFYLKMKGDYYRYLAEVATGEKRATVVESSEKAYSEAHEISKEHMQ PTHPIRLGLALNYSVFYYEIQNAPEQACHLAKTAFDDAIAELDTLNEDSY KDSTLIMQLLRDNLTLWTSDQQDDDGGEGNN
    • Molecular weight
      32 kDa including tags
    • Amino acids
      1 to 247
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab116443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF-TOF
  • Purity
    > 80 % Purified via His tag.

  • Form
    Liquid
  • Additional notes
    ab116443 was alkylated, digested with trypsin and the mass of the resultant peptides determined by MADLI-TOF/TOF. The peptides obtained gave 51 % sequence coverage of 14-3-3 gamma. Theoretical pI: 5.34
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.6% HEPES, 0.02% DTT, 50% Glycerol, 0.012% Benzamidine, 0.003% PMSF

General Info

  • Alternative names
    • 14 3 3 gamma
    • 14 3 3 protein gamma
    • 14 3 3 protein gamma subtype
    • 14 3 3gamma
    • 14-3-3 protein gamma
    • 1433G_HUMAN
    • 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide
    • KCIP 1
    • KCIP-1
    • KCIP1
    • N-terminally processed
    • Protein kinase C inhibitor protein 1
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide
    • Ywhag
    see all
  • Function
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • Tissue specificity
    Highly expressed in brain, skeletal muscle, and heart.
  • Sequence similarities
    Belongs to the 14-3-3 family.
  • Post-translational
    modifications
    Phosphorylated by various PKC isozymes.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • 12 % SDS-PAGE gel showing ab116443.

References

ab116443 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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