Recombinant human Acid Phosphatase protein (ab87493)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIH TAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH

Specifications

Our Abpromise guarantee covers the use of ab87493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity: > 60,000 unit/mg of protein. Unit definition: One unit is defined as the amount of enzyme that will hydrolyze 1nmole of p-nitrophenyl phosphate per minute at 37oC in MES pH5.0 using 10mM of substrate.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    ab87493 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Additional notes
    Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM MES, 2mM EDTA, 0.1mM PMSF, pH 6.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Acid phosphatase 1 soluble
    • Acid phosphatase of erythrocyte
    • ACP1
    • Adipocyte acid phosphatase
    • Cytoplasmic phosphotyrosyl protein phosphatase
    • HAAP
    • LMW-PTP
    • LMW-PTPase
    • Low molecular weight cytosolic acid phosphatase
    • Low molecular weight phosphotyrosine protein phosphatase
    • PAP1
    • PAP2
    • phosphatase, acid, of erythrocyte
    • PPAC_HUMAN
    • Protein tyrosine phosphatase
    • PTPase
    • Purple acid phosphatase
    • Red cell acid phosphatase 1
    • testicular secretory protein Li 37
    see all
  • Function
    Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity.
  • Tissue specificity
    T-lymphocytes express only isoform 2.
  • Sequence similarities
    Belongs to the low molecular weight phosphotyrosine protein phosphatase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing 3µg ab87493 at approximately 20.1kDa.

References

ab87493 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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