Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman
    • Sequence110-476

Specifications

Our Abpromise guarantee covers the use of ab79647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific Activity: 88 pmol/min/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Acetate kinase 1
    • Acetokinase 1
    • ACK
    • ACK 1
    • ACK-1
    • ACK1
    • ACK1_HUMAN
    • Activated Cdc42 associated kinase 1
    • Activated CDC42 kinase 1
    • Activated p21cdc42Hs kinase
    • FLJ44758
    • FLJ45547
    • p21cdc42Hs
    • TNK 2
    • TNK2
    • Tyrosine kinase non receptor 2
    • Tyrosine kinase non receptor protein 2
    • Tyrosine kinase non-receptor protein 2
    see all
  • FunctionNon-receptor tyrosine kinase that regulates the activity of a number of proteins by tyrosine phosphorylation especially proteins critical for cell survival, cell growth, and proliferation. Activates AKT1 by phosphorylating it on 'Tyr-176' resulting in its activation. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' and promotes its recruitment to the androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development.
  • Tissue specificityThe Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
    Contains 1 UBA domain.
  • DomainThe EBD (EGFR-binding domain) domain is necessary for interaction with EGFR.
    The SAM-like domain is necessary for NEDD4-mediated ubiquitination. Promotes membrane localization and dimerization to allow for autophosphorylation.
    The UBA domain binds both poly- and mono-ubiquitin.
  • Post-translational
    modifications
    Autophosphorylation regulates kinase activity. Phosphorylation on Tyr-518 is required for interaction with SRC.
    Ubiquitinated by NEDD4. Its EGF-induced degradation is EGFR activation-dependent and is processed by lysosomes, not proteasomes.
  • Cellular localizationCell membrane. Nucleus. Endosome. Cell junction > adherens junction. Cytoplasmic vesicle membrane. The Tyr-284 phosphorylated form is expressed both in the membrane and nucleus. Co-localizes with EGFR on the endosomes.
  • Information by UniProt

Recombinant human ACK1 protein images

  • ab79647 on SDS-PAGE, Molecular Weight 68 kDa
    10% SDS-PAGE, Coomassie staining
    Lane 1: ab79647 7µg.
    Lane 2: Protein marker.
  • Kinase Activity - Specific Activity 88 pmol/min/mg

References for Recombinant human ACK1 protein (ab79647)

ab79647 has not yet been referenced specifically in any publications.

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