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Our Abpromise guarantee covers the use of ab52101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SPECIFIC ACTIVITY: 300,000-400,000 units/mg UNIT DEFINITION: One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37oC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Active Caspase 3 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. The active Caspase 3 preferentially cleaves Caspase 3 substrates e.g. DEVD-AFC or DEVD-pNA.
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 15% Glycerol, PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ab52101 has not yet been referenced specifically in any publications.