Overview

Description

  • NatureRecombinant
  • SourceBTI-TN-5B1-4 cells
  • Amino Acid Sequence
    • AccessionP08476
    • SpeciesHuman
    • SequenceGLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS
    • Amino acids311 to 426
    • Additional sequence informationThe sequence is the full length mature protein minus signal peptide & propeptide. This protein is Activin A, 26.0 kDa disulfide-linked homodimer of 2 βA chains, each containing 116 aa residues.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab50051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Biological Activity : The ED50 as determined by its ability to inhibit the proliferation of murine MPC-11 cells is < 2 ng/ml, corresponding to a specific activity of > 5 x 105 units/mg.

  • Applications

    Cell differentiation

    SDS-PAGE

    Functional Studies

  • Purity> 95 % SDS-PAGE.
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • FormLyophilised
  • Additional notesThis protein is expressed in (BTI-Tn-5B1-4) High-5 Insect Cells
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    pH: 3.00
    Constituent: 0.2941% Sodium citrate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8oC for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20oC to -80oC.

General Info

  • Alternative names
    • Activin A
    • Activin beta A chain
    • Activin beta-A chain
    • ActivinA
    • EDF
    • Erythroid differentiation factor
    • Erythroid differentiation protein
    • INHBA
    • INHBA_HUMAN
    • Inhibin beta A chain
    see all
  • FunctionInhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted.
  • Information by UniProt

Recombinant human Activin A protein images

  • Human embryonic stem cells (HUES4) were differentiated as monolayer cultures on matrigel coated dishes using a protocol described in Kroon et al, Nat Biotechnol. (2008). Activin A (ab50051) and Wnt3a (ab81484) were added to cultures to direct differentiation towards definitive endoderm. This image was taken 3 days after induction, where the definitive endoderm marker FOXA2 (ab40874 in green) is abundant. Dapi staining is shown in blue. Magnification: 200x. Negative controls of fluorescently conjugated secondary antibodies only showed no staining.

References for Recombinant human Activin A protein (ab50051)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Te pido disculpas por la espera.

He comprobado con mis compañeros del laboratorio que ninguna de las proteínas Inhibina A o B disponibles actualmente en el catalogo corresponde al heterodímero activo.

Sin embarg...

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Thank you for contacting us. The expected molecular weight for Inhibin beta A protein ab50051 is 26.0kDa. Please be advised that ab25747 is not a full length protein it is a peptide which we have used to produce antibody ab24562. Could you p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"