The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20ºC.
Constituents: 0.32% Tris HCl, 0.58% Sodium chloride
Reconstitute with water to desired concentration.
136 kDa double-stranded RNA-binding protein
136kDa double stranded RNA binding protein
Adenosine deaminase acting on RNA 1 A
Adenosine deaminase RNA specific
Adenosine deaminase RNA specific 1
Adenosine deaminase that act on RNA
Double stranded RNA specific adenosine deaminase
Double-stranded RNA-specific adenosine deaminase
Double-stranded RNA-specific editase Adar
dsRNA adenosine deaminase
Interferon induced protein 4
Interferon inducible protein 4
Interferon-inducible protein 4
Pre-mRNA adenosine deaminase
RNA adenosine deaminase 1
RNA-editing deaminase 1
RNA-editing enzyme 1
Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site-selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression.
Ubiquitously expressed, highest levels were found in brain and lung.
Involvement in disease
Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH) [MIM:127400]; also known as reticulate acropigmentation of Dohi. DSH is a pigmentary genodermatosis of autosomal dominant inheritance characterized by a mixture of hyperpigmented and hypopigmented macules distributed on the dorsal parts of the hands and feet.
Contains 1 A to I editase domain. Contains 2 DRADA repeats. Contains 3 DRBM (double-stranded RNA-binding) domains.
Sumoylation reduces RNA-editing activity.
Cytoplasm. Nucleus > nucleolus. Isoform 1 is found predominantly in cytoplasm but appears to shuttle between the cytoplasm and nucleus. Isoform 5 is found exclusively in the nucleolus.