Recombinant human ADBR2 + Gia2 fusion co-expressed with ß1 and ?1 protein (ab90755)

Overview

  • Product nameRecombinant human ADBR2 + Gia2 fusion co-expressed with ß1 and ?1 protein
  • Protein lengthFull length protein
  • Description
    Recombinant human ADBR2 + Giα2 fusion co-expressed with β1 and γ1 protein

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman
    • Additional sequence informationTagged at the N terminus of the ADBR2 with a DDDDK tag. The C terminus of the ADBR2 is linked to the N terminus of the Gi alpha 2, beta 1, gamma 1 with a His tag. 90kDa + 36kDa.

Specifications

Our Abpromise guarantee covers the use of ab90755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityActivity: 3.5-7.7 pmol/mg.
  • Applications

    Functional Studies

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 75mM Tris HCl, 12.5mM Magnesium chloride, 1mM EDTA, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Adenylate cyclase inhibiting G alpha protein
    • ADRB2
    • ADRB2R
    • ADRBR
    • Adrenergic beta 2 receptor surface
    • B2AR
    • BAR
    • beta 2 adrenoceptor
    • Beta 2 adrenoreceptor
    • Beta subunit signal transducing proteins GS/GI
    • BETA2AR
    • Catecholamine receptor
    • G gamma I
    • G protein beta 1 subunit
    • GBB1
    • GIP
    • GNAI 2
    • GNAI2B
    • GNG 2
    • GNG2
    • GTP binding regulatory protein Gi alpha 2 chain
    • Guanine nucleotide binding protein beta 1 subunit
    • Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 2
    • Guanine nucleotide binding protein gamma 2
    • H LUCA15.1
    • H LUCA16.1
    • Transducin beta chain 1
    • WUGSC:H LUCA15.1
    • WUGSC:H LUCA16.1
    see all
  • RelevanceADBR2 is a member of the G protein-coupled receptor superfamily. It is activated by the catecholamine epinephrine and couples to the G protein Gs to mediate adenylate cyclase activation. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. The G protein family of signal transducers includes 5 heterotrimers, which are most clearly distinguished by their different alpha chains; they have virtually identical beta chains and similar gamma chains. G protein alpha inhibitor chain 2 is a subunit of the G(i) G protein, whose role is in inhibiting adenylate cyclase activity in response to bound GTP. The G proteins possess GTPase activity and are components of a complex membrane signaling system that includes membrane bound receptors. The system exists to modulate extracellular signals as they are transmitted into the cell. GNB1 is a beta subunit. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
  • Cellular localizationADBR2 and ?1: Cell Membrane Gia2: Plasma Membrane

References for Recombinant human ADBR2 + Gia2 fusion co-expressed with ß1 and ?1 protein (ab90755)

ab90755 has not yet been referenced specifically in any publications.

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