Recombinant human ADRB2 + GsaL fusion protein (ab90830)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Molecular weight
      104 kDa
    • Additional sequence information
      Tagged at the N terminus of the ADBR2 with a DDDDK tag. The C terminus of the ADBR2 is linked to the N terminus of the Gs alpha L with a His tag.

Specifications

Our Abpromise guarantee covers the use of ab90830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Receptor expression level: 4.8-15.5 pmol/mg
  • Applications

    Functional Studies

  • Form
    Liquid
  • Additional notes
    Receptor expression level: 4.8-15.5 pmol/mg
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 75mM Tris HCl, 12.5mM Magnesium chloride, 1mM EDTA, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Adenylate cyclase stimulating G alpha protein
    • ADRB2
    • ADRB2R
    • ADRBR
    • Adrenergic beta 2 receptor surface
    • AHO
    • B2AR
    • BAR
    • Beta 2 adrenergic receptor
    • Beta 2 adrenoceptor
    • Beta 2 adrenoreceptor
    • BETA2AR
    • C20orf45
    • Catecholamine receptor
    • GNAS
    • GNAS complex locus
    • GNAS1
    • GPSA
    • GSA
    • GSP
    • Guanine nucleotide binding protein (G protein) alpha stimulating activity polypeptide 1
    • Guanine nucleotide binding protein G(s) subunit alpha isoforms short
    • Guanine nucleotide regulatory protein
    • MGC33735
    • PHP1A
    • PHP1B
    • POH
    see all
  • Relevance
    ADRB2 is a member of the G protein-coupled receptor superfamily. It is activated by the catecholamine epinephrine and couples to the G protein Gs to mediate adenylate cyclase activation. ADRB2 binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. GsaL is the long splice variant of the alpha-subunit of the heterotrimeric G-protein Gs. It differs from the short splice variant by a 15 amino acid insert between the ras-like domain and the alpha-helical domain.
  • Cellular localization
    ADRB2: Cell membrane; Multi-pass membrane protein

References

ab90830 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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