Overview

Description

  • Nature
    Recombinant
  • Source
    Wheat germ
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGFDVKDHA QKQETANSLPAPPQMPLPEIPQPWLPPDSGPPPLPTSSLPEGYYEEAVPL SPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDL VKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQG CNITYIPKDSKKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSG CSGPVDSECPPPPSSPVHKAELEKKLSSERPSSDGEGVVENGITTCNGKE QVKRKKSSKSEAKGTVSKVTGKKITKIISLGKKKPSTDEQTSSAEEDVPT CGYLNVLSNSRWRERWCRVKDNKLIFHKDRTDLKTHIVSIPLRGCEVIPG LDCKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPEALH YDYIDVEMSASVIQTAKQTFCFMNRRVISANPYLGGTSNGYAHPSGTALH YDDVPCINGSLKGKKPPVASNGVTGKGKTLSSQPKKADPAAVVKRTGSNA AQYKYGKNRVEADAKRLQTKEEELLKRKEALRNRLAQLRKERKDLRAAIE VNAGRKPQAILEEKLKQLEEECRQKEAERVSLELELTEVKESLKKALAGG VTLGLAIEPKSGTSSPQSPVFRHRTLENSPISSCDTSDTEGPVPVNSAAV LKKSQAAPGSSPCRGHVLRKAKEWELKNGT
    • Amino acids
      1 to 730
    • Tags
      proprietary tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab163708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • Form
    Liquid
  • Additional notes
    Protein concentration is above or equal to 0.05 mg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • 110 kDa actin filament associated protein
    • 110 kDa actin filament-associated protein
    • Actin filament associated protein 1
    • Actin filament associated protein 110 kDa
    • Actin filament-associated protein 1
    • AFAP
    • AFAP 1
    • AFAP 110
    • AFAP-110
    • Afap1
    • AFAP1_HUMAN
    • AFAP110
    • FLJ56849
    see all
  • Function
    Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.
  • Tissue specificity
    Low expression in normal breast epithelial cell line MCF-10A and in tumorigenic breast cancer cell lines MCF-7, T-47D and ZR75-1. Highly expressed in the invasive breast cancer cell lines MDA-MB-231 and MDA-MB-435. Overexpressed in prostate carcinoma.
  • Sequence similarities
    Contains 2 PH domains.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues by SRC.
  • Cellular localization
    Cytoplasm > cytoskeleton. Localizes with stress fibers in quiescent cells, concentrated in cell motility structures such as lamellipodia, filopodia and membrane ruffles upon their induction.
  • Information by UniProt

Images

  • ab163708 on a 12.5% SDS-PAGE stained with Coomassie Blue.

References

ab163708 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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