Recombinant Human AFAP protein (denatured) (ab181922)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human AFAP protein (denatured) -
Purity
> 80 % SDS-PAGE.
ab181922 is purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMGCSGPVDSECPPPPSSPVHKAELEKKLSS ERPSSDGEGVVENGITTCNGKEQVKRKKSSKSEAKGTVSKVTGKKITKII SLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDNKLIFHK DRTDLKTHIVSIPLRGCEVIPGLDCKHPLTFRLLRNGQEVAVLEASSSED MGRWIGILLAETGSSTDPEALHYDYIDVEMSASVIQTAKQTFCFMNRRVI SANPYLGGTSNGYAHPSGTALHYDDVPCINGSLRGKKPPVASNGVTGKGK TLSSQPKKADPAAVVKRTGSNAAQYKYGKNRVEADAKRLQTKEEELLKRK EALRNRLAQLRK -
Predicted molecular weight
40 kDa including tags -
Amino acids
250 to 590 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab181922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 2.4% Urea, 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- 110 kDa actin filament associated protein
- 110 kDa actin filament-associated protein
- Actin filament associated protein 1
see all -
Function
Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells. -
Tissue specificity
Low expression in normal breast epithelial cell line MCF-10A and in tumorigenic breast cancer cell lines MCF-7, T-47D and ZR75-1. Highly expressed in the invasive breast cancer cell lines MDA-MB-231 and MDA-MB-435. Overexpressed in prostate carcinoma. -
Sequence similarities
Contains 2 PH domains. -
Post-translational
modificationsPhosphorylated on tyrosine residues by SRC. -
Cellular localization
Cytoplasm > cytoskeleton. Localizes with stress fibers in quiescent cells, concentrated in cell motility structures such as lamellipodia, filopodia and membrane ruffles upon their induction. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab181922 has not yet been referenced specifically in any publications.