Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab80352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific Activity: The addition of AHA1 increased ATPase activity of hsp90 alpha from 0.18 to 0.94 pmol/min/ug. Assay conditions: 50 µl reaction mix (100 mM Tris-HCl pH7.4, 20 mM KCl, 6 mM MgCl2, 200 µM ATP, and 5 µg Hsp90alpha) was incubated with Aha1 (0-10 µg) for 3 hrs at 37ºC. To stop the reaction, 100 µl of the malachite green reagent was added to each well. Absorbance at 620 nm was measured.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Activator of 90 kDa heat shock protein ATPase homolog 1
    • Activator of heat shock 90kDa protein ATPase homolog 1
    • AHA 1
    • AHA1
    • AHSA 1
    • Ahsa1
    • AHSA1_HUMAN
    • C14orf3
    • HSPC322
    • p38
    see all
  • FunctionCochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking.
  • Tissue specificityExpressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.
  • Sequence similaritiesBelongs to the AHA1 family.
  • Cellular localizationCytoplasm > cytosol. Endoplasmic reticulum. May transiently interact with the endoplasmic reticulum.
  • Information by UniProt

Recombinant human AHA1 protein images

  • Image showing the effect of ab80352 on ATPase activity of HSP90a.
  • 10%SDS-PAGE showing ab80352 at approximately 38kDa (7µg).

References for Recombinant human AHA1 protein (ab80352)

ab80352 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"