Recombinant human AKR1B10 protein (ab168014)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI RFHIQRNVIV IPKSVTPARI VENIQVFDFK LSDEEMATIL SFNRNWRACN VLQSSHLEDY PFDAEY
    • Molecular weight
      36 kDa
    • Amino acids
      1 to 316

Specifications

Our Abpromise guarantee covers the use of ab168014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The specific activity of AKR1B10 with 5 mM glyceraldehyde is approximately ~100 mU/mg. (Specific activity is calculated by the decrease of absorption at 340 nm in presence of 2-10 µg AKR1B10, 5mM glyceraldehyde, 200 µM NADPH in 0.1 M sodium phosphate buffer, pH 7.5).

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    >90% by SDS-PAGE.

  • Form
    Liquid
  • Additional notes
    Centrifuge the vial prior to opening
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: Tris HCl, 50% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • AK1BA_HUMAN
    • AKR1B10
    • AKR1B11
    • AKR1B12
    • Aldo keto reductase family 1 member B10
    • aldo keto reductase family 1 member B11
    • Aldo-keto reductase family 1 member B10
    • Aldo-keto reductase family 1 member B10 (aldose reductase)
    • Aldo-keto reductase family 1 member B11 (aldose reductase-like)
    • Aldose reductase like
    • aldose reductase like 1
    • aldose reductase like peptide
    • Aldose reductase related protein
    • Aldose reductase-like
    • Aldose reductase-related protein
    • ALDRLn
    • ARL 1
    • ARL-1
    • ARL1
    • ARL1 Human small intestine reductase
    • ARP
    • hARP
    • HIS
    • HSI
    • SI reductase
    • Small intestine reductase
    see all
  • Function
    Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs.
  • Tissue specificity
    Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.
  • Sequence similarities
    Belongs to the aldo/keto reductase family.
  • Cellular localization
    Lysosome. Secreted. Secreted through a lysosome-mediated non-classical pathway.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of 3 µg ab168014.

References

ab168014 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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