Recombinant Human AKR1D1 protein (ab125483)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQVECHPYF TQPKLLKFCQ QHDIVITAYS PLGTSRNPIW VNVSSPPLLK DALLNSLGKR YNKTAAQIVL RFNIQRGVVV IPKSFNLERI KENFQIFDFS LTEEEMKDIE ALNKNVRFVE LLMWRDHPEY PFHDEY
    • Molecular weight
      40 kDa including tags
    • Amino acids
      1 to 326
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab125483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 90 % SDS-PAGE.
    ab125483 is purified by proprietary chromatographic techniques.
  • Form
    Liquid
  • Additional notes
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Store under desiccating conditions.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 20% Glycerol, 0.58% Sodium chloride

General Info

  • Alternative names
    • 3-oxo-5-beta-steroid 4-dehydrogenase
    • 3o5bred
    • AK1D1_HUMAN
    • AKR1D1
    • Aldo keto reductase family 1 member D1
    • aldo keto reductase family 1 member D1 (delta 4 3 ketosteroid 5 beta reductase)
    • Aldo-keto reductase family 1 member D1
    • CBAS2
    • Delta(4) 3 ketosteroid 5 beta reductase
    • Delta(4) 3 oxosteroid 5 beta reductase
    • Delta(4)-3-ketosteroid 5-beta-reductase
    • Delta(4)-3-oxosteroid 5-beta-reductase
    • SRD5B1
    • steroid 5 beta reductase
    • steroid 5 beta reductase beta polypeptide 1 (3 oxo 5 beta steroid delta 4 dehydrogenase beta 1)
    see all
  • Function
    Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.
  • Tissue specificity
    Highly expressed in liver. Expressed in testis and weakly in colon.
  • Involvement in disease
    Congenital bile acid synthesis defect 2
  • Sequence similarities
    Belongs to the aldo/keto reductase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

References

ab125483 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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