Recombinant human AKR7A2 protein (ab168001)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELE LSAASRVVSR AAVHCALRSP PPEARALAMS RPPPPRVASV LGTMEMGRRM DAPASAAAVR AFLERGHTEL DTAFMYSDGQ SETILGGLGL GLGGGDCRVK IATKANPWDG KSLKPDSVRS QLETSLKRLQ CPQVDLFYLH APDHGTPVEE TLHACQRLHQ EGKFVELGLS NYASWEVAEI CTLCKSNGWI LPTVYQGMYN ATTRQVETEL FPCLRHFGLR FYAYNPLAGG LLTGKYKYED KDGKQPVGRF FGNSWAETYR NRFWKEHHFE AIALVEKALQ AAYGASAPSV TSAALRWMYH HSQLQGAHGD AVILGMSSLE QLEQNLAATE EGPLEPAVVD AFNQAWHLVA HECPNYFR
    • Molecular weight
      44 kDa including tags
    • Amino acids
      1 to 359
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab168001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity is approximately 0.25 – 0.3 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25°C. Specific activity was expressed as units/mg protein.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    >95% by SDS-PAGE .

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.05% DTT, 0.32% Tris HCl, 20% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • AFAR
    • AFAR1
    • AFB1 aldehyde reductase 1
    • AFB1 AR1
    • AFB1-AR 1
    • AFB1AR1
    • Aflatoxin aldehyde reductase
    • Aflatoxin B1 aldehyde reductase member 2
    • Aflatoxin beta1 aldehyde reductase
    • Aiar
    • AKR7
    • Akr7a2
    • Aldo keto reductase family 7
    • Aldo keto reductase family 7 member A2
    • Aldo keto reductase family 7 member A2 aflatoxin aldehyde reductase
    • Aldo keto reductase family 7, member A2 (aflatoxin aldehyde reductase)
    • Aldoketoreductase 7
    • ARK72_HUMAN
    • SSA reductase
    • Succinic semialdehyde reductase
    see all
  • Function
    Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.
  • Tissue specificity
    Detected in brain, liver, small intestine and testis, and at lower levels in heart, prostate, skeletal muscle and spleen. Detected in kidney proximal and distal tubules, endothelial cells lining the Bowman's capsules and some cysts. Detected at low levels in lung and pancreas (at protein level). Widely expressed.
  • Sequence similarities
    Belongs to the aldo/keto reductase 2 family.
  • Cellular localization
    Golgi apparatus. Cytoplasm.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab168001 (3 µg).

References

ab168001 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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