Recombinant Human AKR7A2 protein (ab93634)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM LSAASRVVSR AAVHCALRSP PPEARALAMS RPPPPRVASV LGTMEMGRRM DAPASAAAVR AFLERGHTEL DTAFMYSDGQ SETILGGLGL GLGGGDCRVK IATKANPWDG KSLKPDSVRS QLETSLKRLQ CPQVDLFYLH APDHGTPVEE TLHACQRLHQ EGKFVELGLS NYASWEVAEI CTLCKSNGWI LPTVYQGMYN ATTRQVETEL FPCLRHFGLR FYAYNPLAGG LLTGKYKYED KDGKQPVGRF FGNSWAETYR NRFWKEHHFE AIALVEKALQ AAYGASAPSV TSAALRWMYH HSQLQGAHGD AVILGMSSLE QLEQNLAATE EGPLEPAVVD AFNQAWHLVA HECPNYFR

Specifications

Our Abpromise guarantee covers the use of ab93634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.
    ab93634 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • AFAR
    • AFAR1
    • AFB1 aldehyde reductase 1
    • AFB1 AR1
    • AFB1-AR 1
    • AFB1AR1
    • Aflatoxin aldehyde reductase
    • Aflatoxin B1 aldehyde reductase member 2
    • Aflatoxin beta1 aldehyde reductase
    • Aiar
    • AKR7
    • Akr7a2
    • Aldo keto reductase family 7
    • Aldo keto reductase family 7 member A2
    • Aldo keto reductase family 7 member A2 aflatoxin aldehyde reductase
    • Aldo keto reductase family 7, member A2 (aflatoxin aldehyde reductase)
    • Aldoketoreductase 7
    • ARK72_HUMAN
    • SSA reductase
    • Succinic semialdehyde reductase
    see all
  • FunctionCatalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.
  • Tissue specificityDetected in brain, liver, small intestine and testis, and at lower levels in heart, prostate, skeletal muscle and spleen. Detected in kidney proximal and distal tubules, endothelial cells lining the Bowman's capsules and some cysts. Detected at low levels in lung and pancreas (at protein level). Widely expressed.
  • Sequence similaritiesBelongs to the aldo/keto reductase 2 family.
  • Cellular localizationGolgi apparatus. Cytoplasm.
  • Information by UniProt

Recombinant Human AKR7A2 protein images

  • 15% SDS-PAGE showing ab93634 at approximately 44kDa (3µg).

References for Recombinant Human AKR7A2 protein (ab93634)

ab93634 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot

Review text: This human AKR7A2 recombinant protein works just as you would want it to. We used this protein to confirm the reactivity and sensitivity of the AKR7A2 polyclonal antibody (ab97458). See blot image below!
Sample: Human Tissue lysate - whole (Heart)
Loading amount: 0.03 µg
Specification: Heart
Gel Running Conditions: Non-reduced Denaturing (12% Tris/glycine gel)
Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details
Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS-Tween 20 (0.05%)

Primary antibody (in addition to 'AKR7A2 protein (His tag)')
Dilution: 1/1000

Secondary antibody
Name: Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection
Detection method: ECL
Exposure: 1 minute(s) and 0 second(s)
Bands: Specific: 40 kDa
Positive control: No positive control was used since this AKR7A2 recombinant protein was our protein of interest.

Additional data
Additional Notes: Nitrocellulose membranes were used in our WB. The image of the standard curve shows the CBR3 recombinant protein in increasing amounts from left to right (0.03, 0.07, and 0.10 ug) and a protein ladder on the far right as detected using the conditions outlined above.
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Submitted Nov 28 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"