Recombinant human AKR7A3 protein (ab116489)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM SRQLSRARPA TVLGAMEMGR RMDAPTSAAV TRAFLERGHT EIDTAFVYSE GQSETILGGL GLRLGGSDCR VKIDTKAIPL FGNSLKPDSL RFQLETSLKR LQCPRVDLFY LHMPDHSTPV EETLRACHQL HQEGKFVELG LSNYAAWEVA EICTLCKSNG WILPTVYQGM YNAITRQVET ELFPCLRHFG LRFYAFNPLA GGLLTGKYKY EDKDGKQPVG RFFGNTWAEM YRNRYWKEHH FEGIALVEKA LQAAYGASAP SMTSATLRWM YHHSQLQGAH GDAVILGMSS LEQLEQNLAA AEEGPLEPAV VDAFNQAWHL VAHECPNYFR
    • Molecular weight
      42 kDa including tags
    • Amino acids
      1 to 331
    • Additional sequence information
      AKR7A3 Human Recombinant fused to 39 amino acid His Tag at N-terminal produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 370 amino acids (1-331 a.a.).

Specifications

Our Abpromise guarantee covers the use of ab116489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity: approximately < 0.1 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25C. Specific activity was expressed as units/mg protein.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 95 % SDS-PAGE.
    ab116489 was purified by proprietary chromatographic techniques and filter sterilized.
  • Form
    Liquid
  • Additional notes
    although stable at 4°C for 30 days, should be stored desiccated below -20°C for periods greater than 30 days. Please avoid freeze-thaw cycles.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Please see notes section.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 0.58% Sodium chloride, 10% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • AFAR 2
    • AFAR2
    • AFB1 aldehyde reductase 2
    • AFB1 AR 2
    • AFB1-AR 2
    • Aflatoxin aldehyde reductase
    • Aflatoxin B1 aldehyde reductase 2
    • Aflatoxin B1 aldehyde reductase member 3
    • Akr7a3
    • Aldo keto reductase family 7 member A3
    • ARK73_HUMAN
    • OTTHUMP00000002623
    see all
  • Function
    Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.
  • Tissue specificity
    Expressed in colon, kidney, liver, pancreas, adenocarcinoma and endometrium.
  • Sequence similarities
    Belongs to the aldo/keto reductase 2 family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

References for Recombinant human AKR7A3 protein (ab116489)

ab116489 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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