This product is an active protein and may elicit a biological response in vivo, handle with caution.
Alanine tRNA ligase
Alanine tRNA ligase 1, cytoplasmic
Alanine tRNA ligase cytoplasmic
Alanyl tRNA synthetase
Alanyl tRNA synthetase cytoplasmic
Renal carcinoma antigen NY REN 42
Renal carcinoma antigen NY-REN-42
FunctionCatalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
Involvement in diseaseCharcot-Marie-Tooth disease 2N
Sequence similaritiesBelongs to the class-II aminoacyl-tRNA synthetase family.
DomainConsists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs. The C-terminal C-Ala domain (residues 756 to 968), along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs. The human domain can be used in vitro to replace the corresponding domain in E.coli.