Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman
    • Molecular weight108 kDa
    • Additional sequence informationAlanyl-tRNA synthetase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 107,598 Dalton

Specifications

Our Abpromise guarantee covers the use of ab73442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityab73442 is reactive against Polymyositis sera.
  • Applications

    Western blot

    ELISA

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.
    ab73442 is purified by proprietary chromatographic techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 250mM Sodium chloride, 16mM HEPES, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • AARS
    • AI316495
    • Alanine tRNA ligase
    • Alanine tRNA ligase 1, cytoplasmic
    • Alanine tRNA ligase cytoplasmic
    • Alanine--tRNA ligase
    • Alanyl tRNA synthetase
    • Alanyl tRNA synthetase cytoplasmic
    • Alanyl-tRNA synthetase
    • AlaRS
    • C76919
    • CMT2N
    • cytoplasmic
    • EC 6.1.1.7
    • MGC37368
    • Renal carcinoma antigen NY REN 42
    • Renal carcinoma antigen NY-REN-42
    • SYAC_HUMAN
    see all
  • FunctionCatalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
  • Involvement in diseaseCharcot-Marie-Tooth disease 2N
  • Sequence similaritiesBelongs to the class-II aminoacyl-tRNA synthetase family.
  • DomainConsists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.
    The C-terminal C-Ala domain (residues 756 to 968), along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs. The human domain can be used in vitro to replace the corresponding domain in E.coli.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localizationCytoplasm.
  • Information by UniProt

References for Recombinant human AlaRS protein (ab73442)

ab73442 has not yet been referenced specifically in any publications.

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