Recombinant human ALDH2 protein (ab87415)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S
    • Molecular weight
      55 kDa
    • Amino acids
      18 to 517

Specifications

Our Abpromise guarantee covers the use of ab87415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific activity is > 250pmol/min/ug, and was obtained by measuring the increase of NADH in absorbance at 340 nm resulting from the reduction of NAD at pH 8.0 at 25C.

     

     

  • Applications

    SDS-PAGE

    Functional Studies

    Western blot

  • Endotoxin level
    < 1.000 Eu/mg
  • Purity
    > 90 % SDS-PAGE.
    ab87415 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Additional notes
    Endotoxin:< 1.0 EU per 1 µg of protein (determined by LAL method).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM EDTA, 1mM DTT, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Acetaldehyde dehydrogenase 2
    • Aldehyde dehydrogenase 2 family
    • Aldehyde dehydrogenase 2 family (mitochondrial)
    • Aldehyde dehydrogenase mitochondrial
    • Aldehyde dehydrogenase, mitochondrial
    • ALDH 2
    • ALDH class 2
    • ALDH E2
    • ALDH-E2
    • Aldh2
    • ALDH2_HUMAN
    • ALDHI
    • ALDM
    • Liver mitochondrial ALDH
    • MGC1806
    • Mitochondrial aldehyde dehydrogenase 2
    • MS767
    • Nucleus encoded mitochondrial aldehyde dehydrogenase 2
    see all
  • Pathway
    Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
  • Sequence similarities
    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt

Recombinant human ALDH2 protein images

  • 15% SDS-PAGE showing 3µg ab87415 at approximately 54.5 kDa.
  • All lanes : Anti-ALDH2 antibody (ab70917) at 1/100 dilution

    Lane 1 : Natural human Alpha 2 Antiplasmin protein (ab77936) at 0.1 µg
    Lane 2 : Natural human Alpha 2 Antiplasmin protein (ab77936) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Recombinant human ALDH2 protein (ab87415)

ab87415 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply. The specific information activity for these products is as follows:


ab87415: Specific activity is > 0.14 units/ml

ab93463 and ab117154: Specific activity is > 0.5 units/ml

The protocol used...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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