The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific activity is > 0.5 units/ml, and was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD. One unit will oxidize 1.0 µmole of acetaldehyde to acetic acid per minute at pH 8.0 at 25°C in the presence of beta-NAD, potassium and thiols.
>95% by SDS-PAGE .
Centrifuge the vial prior to opening
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes.
High levels in stomach, esophagus and lung; low level in the liver and kidney.